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À¯ÀüÀÚ, º¤ÅÍ, ±¸Á¶Ã¼, Ŭ·ÐÀÇ °³¹ß ´Ù¿î½ºÆ®¸²¿¡¼ÀÇ ¼º°øÀ¸·Î ¿¬°áµÇ´Â ¾÷½ºÆ®¸²¿¡¼ÀÇ ÆÇ´Ü1¿ù 9ÀÏ¡10ÀÏ »ý¹°ÇÐÀû ¿ä¹ý¿¡ À̿밡´ÉÇÑ °íǰÁú ´Ü¹éÁú¿¡ ´ëÇÑ ¼ö¿ä°¡ Àü·Ê¾ø´Â ±Ô¸ð·Î È®´ëµÇ°í ÀÖ½À´Ï´Ù. ±â´É¼º ´Ü¹éÁúÀÇ »ý»êÀ» °ú°Å ¾î´À¶§º¸´Ù ³ôÀº ¼öÁØÀ¸·Î ²ø¾î¿Ã¸°´Ù´Â ¸ñÇ¥¸¦ ´Þ¼ºÇϱâ À§Çؼ´Â °øÇÐ(°úÇÐ)°ú ÅëÂû(¹æ¹ý·Ð)ÀÌ ÇÊ¿äÇÕ´Ï´Ù. »ý¹°¿ä¹ý¿ë ´Ü¹éÁúÀÇ È¿°úÀûÀÎ ¹ßÇöÀ̶ó´Â °úÁ¦´Â °úÇаú ¹æ¹ý·Ð ¾çÂÊ ¸ðµÎ¿¡ °ü·ÃµÈ Å׸¶ÀÔ´Ï´Ù. ÀÌ ºÎȸ´Â »ý¹°¿ä¹ý¿ë ±â´É¼º ´Ü¹éÁú Á¦Ç°À¸·Î ¿¬°áµÇ´Â ´Ü¹éÁú ¹ß°ß ¿¬±¸¿¡ ´Ü¹éÁú °øÇÐÀÇ È¿°úÀûÀÎ Àü·«À» ÀÀ¿ëÇÑ´Ù´Â Àüȸ±îÁöÀÇ Å׸¶¸¦ ´ä½ÀÇÑ °ÍÀ¸·Î, °æÇèÀÌ Ç³ºÎÇÑ ¿¬±¸ÀÚ°¡ Á¦°øÇÏ´Â ÇöÀå °æÇè ¹× ÀÀ¿ë ±â¼ú, ¿¬±¸ ¼º°ú µîÀ» ÅëÇØ ¸¹Àº Á¡À» ¹è¿ï ¼ö ÀÖ½À´Ï´Ù. À¯ÀüÀÚÀÇ Ç¥ÀûÈ Efficient Optimization of Protein Expression Using High-Throughput Gene Synthesis and Screens Jingdong Tian, Ph.D., Assistant Professor, Biomedical Engineering, Duke University Semi-Automatic Cloning and Expression of Immunoglobulin Genes from Single-Plasma Cells for High-Throughput Generation of Monoclonal Antibodies Masaharu Isobe, Ph.D., Professor, Molecular and Cellular Biology, Life Sciences and Bioengineering, University of Toyama
º¤ÅÍÀÇ ±¸¼º The Use of a Combinatorial Recombinational Cloning Platform to Simplify Construction of Expression Vectors for Wide-Ranging Areas of Proteomic and Genetic Research Dominic Esposito, Ph.D., Interim Director, Protein Expression Laboratory; Group Leader, Clone Optimization Group, Advanced Technology Program, SAIC-Frederick, Inc. Parallel Construct Expression Evaluation Using a High-Throughput Process for Baculovirus-Infected Insect Cells W. Clay Brown, Ph.D., High-Throughput Protein Lab, Center for Structural Biology, Life Sciences Institute, University Re-Engineering Multicloning Sites for Function and Convenience Hal Alper, Ph.D., Assistant Professor, Chemical Engineering, University of Texas at Austin So Many Options, So Little Money: How to Choose the Right Vector and Screen for Functional Proteins Bill Gillette, Ph.D., Senior Scientist, Group Leader, Protein Expression Laboratory, Advanced Technology Program, SAIC-Frederick, Inc.
¹ßÇö°èÀÇ °³¹ß Maximizing Recombinant Protein Expression Through Systematic Gene Design Mark Welch, Director, Gene Design, DNA2.0 Engineering and Evaluation of Mammalian Secreted Protein Expression Library for High-Throughput Target Discovery Tadas Panavas, Ph.D., Senior Scientist, Molecular and Protein Biosciences, Centocor R&D, Inc. Development of Expression-Ready Constructs for Generation of Proteomic Libraries Susan Celniker, Ph.D., Co-Director, Life Sciences, Lawrence Berkeley National Laboratory Driving a Variant of the Methanol-Inducible AOX1 Promoter from Pichia pastoris with Glycerol for High-Level Expression of (Therapeutic) Proteins Roland Weis, Ph.D., Head, Operations, VTU Technology GmbH, Austria Influence of Histidine Affinity Tags on Protein Structural Order Evelina Angov, M.S., Ph.D., Microbiologist, Malaria Vaccine Development, Walter Reed Army Institute of Research |